The most frequently used detector for AUC, an absorbance optical system (i., a double-beam spectrophotometer), is considered the easiest to use. us parts of the Beckman Optima XL-A/I AUC are discussed. You may nd it useful to read Chapter 2 of the PhD thesis Analytical Ultracentrifugation of Inorganic Colloids for a. The Optima AUC can be fitted with both or either an absorbance module (ABS) to monitor biomolecules that have absorptive signatures between 170 and 800 nm, and a Rayleigh Interference module (INT) to monitor sedimentation differences between the sample and reference solutions. This allows for the. A wide range of AUC-based methods are available for the analysis of interactions, oligomerization, composition, aggregation, membrane proteins, conformational changes, etc. “For the first time, these advanced features enable revolutionary new multi-wavelength experiments, an.
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